Difference between revisions of "Part:BBa J70622"
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<partinfo>BBa_J70622 short</partinfo>
 
<partinfo>BBa_J70622 short</partinfo>
  
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This is a RFC12 compatible cMyc tag meant for N-terminal fusions. Its design is identical to <partinfo>T2007</partinfo>.
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This is a RFC12 compatible version of <partinfo>BBa_T2007</partinfo>.  It is a cMyc tag head domain meant for N-terminal fusions.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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cMyc tags are commonly used for detection purposes but not usually purity due to the non-specificity of the antibodies used.
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Common tag information:
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<ul>
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<li>Protein Sequence: EQKLISEEDL </li>
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<li>Matrix: Monoclonal Antibody</li>
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<li>Yield/Purity: Fairly low yields and commonly low purity</li>
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<li># Steps: One step</li>
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<li>Fusion Location: N-terminus (C-terminus is <partinfo>J70623</partinfo>)</li>
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<li>Affects Function?: Possible, but unlikely</li>
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</ul>
  
 
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Revision as of 15:31, 9 August 2010

RFC12 cMyc Tag Head Domain

This is a RFC12 compatible version of BBa_T2007. It is a cMyc tag head domain meant for N-terminal fusions.

Usage and Biology

cMyc tags are commonly used for detection purposes but not usually purity due to the non-specificity of the antibodies used.

Common tag information:

  • Protein Sequence: EQKLISEEDL
  • Matrix: Monoclonal Antibody
  • Yield/Purity: Fairly low yields and commonly low purity
  • # Steps: One step
  • Fusion Location: N-terminus (C-terminus is BBa_J70623)
  • Affects Function?: Possible, but unlikely

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]