Part:BBa_T2007
c-Myc head domain (EQKLISEEDL)
This is a c-Myc tag head domain designed to be at the N-terminus of a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
Usage and Biology
The c-myc tag can be used at the N- or C-terminus or even internal to proteins. The c-myc-tag has been used in western blots, immunoprecipitation, flow cytometry, and affinity purification. Proteins with the c-myc-tag can be affinity-purified by coupling the murin anti-c-myc antibody 9E10 to divinyl sulphone -activated agarose. Note that the low pH elution conditions used during affinity purification may impact protein properties and that the matrix can not be reused efficiently. Thus, c-myc-tags are more commonly used for protein detection rather than purification.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//proteindomain/head
| None |