Difference between revisions of "Part:BBa K157041"
 
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Transfectionvector with CMV promoter and CFP as reporter. This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
 
Transfectionvector with CMV promoter and CFP as reporter. This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
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*'''[[User:Rshetty|RS]] 23:57, 8 December 2009 (UTC)''': This part has been split into two parts: a plasmid backbone (this part, BBa_K157041) and a part (the insert, BBa_K157045).  The physical DNA needs to be remapped to BBa_K157041-K157045.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:01, 9 December 2009

Transfection vector (similar to pSB1AK8)

Transfectionvector with CMV promoter and CFP as reporter. This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

  • RS 23:57, 8 December 2009 (UTC): This part has been split into two parts: a plasmid backbone (this part, BBa_K157041) and a part (the insert, BBa_K157045). The physical DNA needs to be remapped to BBa_K157041-K157045.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 3405
    Illegal XbaI site found at 3420
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444