Difference between revisions of "Part:pSB1AK8"
 
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<partinfo>BBa_J52017 short</partinfo>
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<partinfo>pSB1AK8 short</partinfo>
  
 
This vector is BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying '''ampicillin''' and '''kanamycin''' resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
 
This vector is BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying '''ampicillin''' and '''kanamycin''' resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
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*'''[[User:Rshetty|RS]] 03:20, 9 December 2009 (UTC)''': This plasmid backbone is not fully compliant with Assembly standard 10.  It is missing a C in the BioBrick suffix.  The NotI and PstI sites overlap as the sequence is currently specified in the Registry.  It is not known whether this error is simply a user entry error.  Or if the deletion is present in the physical DNA as well.
  
 
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The original part is now pSB1AK8-BBa_P1010.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_J52017 SequenceAndFeatures</partinfo>
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<partinfo>pSB1AK8 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_J52017 parameters</partinfo>
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<partinfo>pSB1AK8 parameters</partinfo>
 
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Latest revision as of 03:20, 9 December 2009

Eukaryotic terminator vector (formerly BBa_J52017)

This vector is BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

  • RS 03:20, 9 December 2009 (UTC): This plasmid backbone is not fully compliant with Assembly standard 10. It is missing a C in the BioBrick suffix. The NotI and PstI sites overlap as the sequence is currently specified in the Registry. It is not known whether this error is simply a user entry error. Or if the deletion is present in the physical DNA as well.
  • RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now pSB1AK8-BBa_P1010.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3405
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444