Difference between revisions of "Part:BBa K5117036"
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<ul>
 
<ul>
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<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117021">(BBa_K5117021)</a></html>, </i>
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<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117021">(BBa_K5117021)</a></html> </li>
  
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<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html> </i>
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<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html> </li>
  
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<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4), </i>
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<li><i>eglS</li> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4), </i>
  
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addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 <html><a href="https://parts.igem.org/Part:BBa_K5117024">(BBa_K5117024)</a></html> </i>
+
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 <html><a href="https://parts.igem.org/Part:BBa_K5117024">(BBa_K5117024)</a></html> </li>
  
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<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html> </i>
+
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html> </li>
  
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<li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html> </i>
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<li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html> </li>
 
</ul>
 
</ul>
  

Revision as of 15:01, 1 October 2024


PcotYZ-BsRBS-BsEglS-L2-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
  • ribosome binding site of Bacillus subtilis (BBa_K5117000)
  • eglS</li> gene of <i>Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4), </i> addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117024)
  • cotY gene of Bacillus subtilis (BBa_K5117022)
  • bidirectional terminator B0014 (BBa_B0014)

    • </ul>


    Biosafety level: S1

    Target organism: Bacillus subtilis

    Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display)

    Application: Degradation of cellulose


    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 1696
      Illegal AgeI site found at 726
    • 1000
      COMPATIBLE WITH RFC[1000]