Difference between revisions of "Part:BBa K5117040"
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| − | < | + | <b>Biosafety level:</b> S1 |
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| + | <b>Target organism:</b> <i>Bacillus subtilis</i> | ||
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| + | <b>Main purpose of use:</b> Immobilization of BhBglA on the spore crust of <i>B. subtilis</i> (spore surface display) | ||
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| + | <b>Application:</b> Degradation of cellobiose | ||
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| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K5117040 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5117040 SequenceAndFeatures</partinfo> | ||
Revision as of 14:48, 1 October 2024
PcotYZ-BsRBS-BhBglA-L3-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021),
- ribosome binding site of Bacillus subtilis (BBa_K5117000),
- bglA gene of Bacillus halodurans encoding a beta-glucosidase (EC 3.2.1.21),
- cotY gene of Bacillus subtilis (BBa_K5117022),
- bidirectional terminator B0014 (BBa_B0014).
addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117028),
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Immobilization of BhBglA on the spore crust of B. subtilis (spore surface display)
Application: Degradation of cellobiose
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 764
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1597
- 1000COMPATIBLE WITH RFC[1000]