Difference between revisions of "Part:BBa K5143027:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The Venus/Ruby plasmid is composed of the Venus composite part <a href="https://parts.igem.org/Part:BBa_K5143026" target="_blank">BBa_K5143026</a>, the mRuby2 composite part <a href="https://parts.igem.org/Part:BBa_K5143021" target="_blank">BBa_K5143021</a> and the pUC57 Backbone <a href="https://parts.igem.org/Part:BBa_K5143005" target="_blank">BBa_K5143005</a>. The sequence of the Venus composite part and the mRuby2 composite part have been optimised for the expression in <i> S. cerevisiae </i>
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===Source===
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===References===
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Latest revision as of 09:40, 28 September 2024


pUC57-pGAP-AF_V1-Venus-ENO1_term-p_ADH1-AGA2-mRuby2-6xHis-TDH1_term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 4360
    Illegal EcoRI site found at 7799
    Illegal EcoRI site found at 8326
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7799
    Illegal EcoRI site found at 8326
    Illegal SpeI site found at 4361
    Illegal PstI site found at 4375
    Illegal NotI site found at 4368
    Illegal NotI site found at 8332
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7799
    Illegal EcoRI site found at 8326
    Illegal XhoI site found at 5964
    Illegal XhoI site found at 7805
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 4361
    Illegal EcoRI site found at 7799
    Illegal EcoRI site found at 8326
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 8326
    Illegal EcoRI site found at 7799
    Illegal SpeI site found at 4361
    Illegal PstI site found at 4375
    Illegal NgoMIV site found at 2077
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2727
    Illegal SapI.rc site found at 5073


Design Notes

The Venus/Ruby plasmid is composed of the Venus composite part BBa_K5143026, the mRuby2 composite part BBa_K5143021 and the pUC57 Backbone BBa_K5143005. The sequence of the Venus composite part and the mRuby2 composite part have been optimised for the expression in S. cerevisiae