Difference between revisions of "Part:BBa K5349005:Design"
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===Design Notes=== | ===Design Notes=== | ||
For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Twist Bioscience's DNA synthesis service | For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Twist Bioscience's DNA synthesis service | ||
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===Source=== | ===Source=== | ||
| − | *UL23 : Refer to | + | *UL23 : Refer to [https://parts.igem.org/Part:BBa_K5349000:Design K5349000] |
| − | *sfGFP : Refer to | + | |
| + | *sfGFP : Refer to [https://parts.igem.org/Part:BBa_K2553008:Design K2553008] | ||
===References=== | ===References=== | ||
Latest revision as of 09:20, 26 September 2024
UL23-sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1611
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 879
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Twist Bioscience's DNA synthesis service
Source
- UL23 : Refer to K5349000
- sfGFP : Refer to K2553008