Difference between revisions of "Part:BBa K5349005:Design"

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===Source===
 
  
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UL23 : <a href="https://parts.igem.org/Part:BBa_K5349000:Design" target="_blank">UL23: Refer to K5349000</a>
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===Source===
  
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sfGFP : <a href="https://parts.igem.org/Part:BBa_K2553008:Design" target="_blank">sfGFP: Refer to K2553008</a>
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*UL23 : Refer to [https://parts.igem.org/Part:BBa_K5349000:Design K5349000]
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*sfGFP : Refer to [https://parts.igem.org/Part:BBa_K2553008:Design K2553008]
  
 
===References===
 
===References===

Latest revision as of 09:20, 26 September 2024


UL23-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1611
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Twist Bioscience's DNA synthesis service



Source

References