Difference between revisions of "Part:BBa K5205001"
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<partinfo>BBa_K5205001 short</partinfo> | <partinfo>BBa_K5205001 short</partinfo> | ||
− | + | The Non sequence is an artificially designed signal-like sequence that directs newly synthesized proteins to the cell membrane via a non-classical secretion pathway (Jeiranikhameneh et al., 2017). | |
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− | <span class='h3bb'> | + | <span class='h3bb'></span> |
+ | ===Sequence and Features=== | ||
<partinfo>BBa_K5205001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5205001 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K5205001 parameters</partinfo> | <partinfo>BBa_K5205001 parameters</partinfo> | ||
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+ | <!-- Add more about the biology of this part here --> | ||
+ | ===Usage and Biology=== | ||
+ | The Non signal-like sequence always combined with OmpA a part of the outer membrane protein A (OmpA) [https://parts.igem.org/Part:BBa_K5205002 BBa_K5205002] to form the Non-OmpA system [https://parts.igem.org/Part:BBa_K5205003 BBa_K5205003]. This system efficiently displays fusion proteins on the cell surface in E. coli (Jeiranikhameneh et al., 2017). | ||
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+ | ==References== | ||
+ | Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0 |
Latest revision as of 02:46, 24 September 2024
Non, signal-like sequence
The Non sequence is an artificially designed signal-like sequence that directs newly synthesized proteins to the cell membrane via a non-classical secretion pathway (Jeiranikhameneh et al., 2017).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The Non signal-like sequence always combined with OmpA a part of the outer membrane protein A (OmpA) BBa_K5205002 to form the Non-OmpA system BBa_K5205003. This system efficiently displays fusion proteins on the cell surface in E. coli (Jeiranikhameneh et al., 2017).
References
Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0