Difference between revisions of "Part:BBa K243010"
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<partinfo>BBa_K243010 short</partinfo>
 
<partinfo>BBa_K243010 short</partinfo>
  
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These part units some of our parts. The combination of a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157011 Histidin-Tag], a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157004 Anticalin-Tag] and the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157009 linked] protein domain Fok_a, makes it possible to purifier and detect the function of the protein. The [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157004 fluoresceinA tag] allows the measurement by quenching and the coupling to an flurescein linked oligo.
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These part units some of our parts. The combination of a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157011 Histidin-Tag], a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157004 Anticalin-Tag] and the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157009 linked] protein domain Fok_a, makes it possible to purifier and detect the function of the protein. The [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157004 fluoresceinA tag] allows the measurement by quenching and the coupling to an flurescein linked oligo.<br>
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===Purification===
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We achieved to purify one part of our universal endonuclease by a Histidin column purification and proved it with a following Western Blot.
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[Image:WB09.10.02jpg.jpg] 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:34, 20 October 2009

His-FluA-Split Linker-Fok_i

These part units some of our parts. The combination of a Histidin-Tag, a Anticalin-Tag and the linked protein domain Fok_a, makes it possible to purifier and detect the function of the protein. The fluoresceinA tag allows the measurement by quenching and the coupling to an flurescein linked oligo.

Purification

We achieved to purify one part of our universal endonuclease by a Histidin column purification and proved it with a following Western Blot. [Image:WB09.10.02jpg.jpg]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]