Difference between revisions of "Part:BBa K243010"
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<partinfo>BBa_K243010 short</partinfo>
 
<partinfo>BBa_K243010 short</partinfo>
  
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These part units some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain foka, make it possible to purifier and detect the function of the protein in one step. The fluorescineA tag allows the measurement by quenching and the coupling to an anticalin linked oligo.
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These part units some of our parts. The combination of a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157011 Histidin-Tag], a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157004]anticalin-Tag and the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157009linked] protein domain Fok_a, makes it possible to purifier and detect the function of the protein. The fluorescineA tag allows the measurement by quenching and the coupling to an fluresceine linked oligo.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:34, 20 October 2009

His-FluA-Split Linker-Fok_i

These part units some of our parts. The combination of a Histidin-Tag, a [1]anticalin-Tag and the [2] protein domain Fok_a, makes it possible to purifier and detect the function of the protein. The fluorescineA tag allows the measurement by quenching and the coupling to an fluresceine linked oligo.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]