Difference between revisions of "Part:BBa K203114"
(→Functional Parameters) |
(→Functional Parameters) |
||
| Line 16: | Line 16: | ||
<partinfo>BBa_K203114 parameters</partinfo> | <partinfo>BBa_K203114 parameters</partinfo> | ||
| − | pPARγ was induced by 5µM Thiazolidinedione in [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#U2-OS U2OS cells]. Promoter activtiy was then roughly characterized analogous [http://2009.igem.org/Team:Heidelberg/Measurement REU] by TECAN (automated plate fluorescence reader). This promoter corresponds to clone γL9. (For more accurately characterized promoters created by RA-PCR, see [[Part:BBa_K203119]], [[Part:BBa_K203111]], [[Part:BBa_K203110]], and others) | + | pPARγ was induced by 5µM Thiazolidinedione in [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#U2-OS U2OS cells]. Promoter activtiy was then roughly characterized analogous to [http://2009.igem.org/Team:Heidelberg/Measurement REU] by TECAN (automated plate fluorescence reader). This promoter corresponds to clone γL9. (For more accurately characterized promoters created by RA-PCR, see [[Part:BBa_K203119]], [[Part:BBa_K203111]], [[Part:BBa_K203110]], and others) |
[[Image:HD09_ppary.png|thumb|none|400px|'''Characterization of two pPARγ responsive promoters.''' Clones created by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR] were screened and two promising clones were characterized by triple TECAN reads.]] | [[Image:HD09_ppary.png|thumb|none|400px|'''Characterization of two pPARγ responsive promoters.''' Clones created by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR] were screened and two promising clones were characterized by triple TECAN reads.]] | ||
<!-- --> | <!-- --> | ||
Revision as of 10:20, 17 October 2009
PPARγ-regulated promoter 1
A synthetic promoter upregulated by pPARγ activation. Manufactured by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR].
Usage and Biology
Peroxisome proliferator-activated receptor γ (PPAR γ) is a transcription factor belonging to the family of nuclear receptors. PPAR γ plays an important role in glucose metabolism and fatty acid storage. PPAR γ is basically activated by ligands like the prostaglandin PGJ2 and through dimerization with retinoid X receptor (RXR). The activated heterodimer binds to the DNA consensus sequence AGGTCANAGGTCA resulting in an increased or decreased transcription of the appropriate gene. The genes activated by PPAR γ initiate the uptake of fatty acids and differentiation of cells to adipocytes. Besides its function in metabolism, PPAR γ was also shown to be correlated with several diseases such as cancer and diabetes. Activation of PPAR γ by synthetic PPAR γ ligands result in an increased glucose uptake. These syntethtic ligands are therefore promising agents in diabetes II treatment. Another synthetic ligand of PPAR γ is able to inhibit the proliferation of different cancer types. For references, see [http://2009.igem.org/Team:Heidelberg/Eucaryopedia Heidelberg 2009's Eukaryopedia].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
pPARγ was induced by 5µM Thiazolidinedione in [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#U2-OS U2OS cells]. Promoter activtiy was then roughly characterized analogous to [http://2009.igem.org/Team:Heidelberg/Measurement REU] by TECAN (automated plate fluorescence reader). This promoter corresponds to clone γL9. (For more accurately characterized promoters created by RA-PCR, see Part:BBa_K203119, Part:BBa_K203111, Part:BBa_K203110, and others)
