Difference between revisions of "Part:BBa K4323000"

Latest revision as of 20:42, 13 October 2022

Coding sequence for Aerolysin

Secreted, cytolytic toxin that forms pores in host membranes after proteolytic removal of a C-terminal propeptide, leading to destruction of the membrane permeability barrier and host cell death. The pores are formed by transmembrane beta-strands and are approximately 3 nm in diameter. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. The composite part BBa_K4323001 includes the necessary components to express Aerolysin toxin. For more information, please visit our Wiki page.

Figure 1. The toxicity of Aerolysin to Dreissena bugensis.

Figure 2. His-Trap Ni-column purified Aerolysin. L: ladder, C5: 10 % B wash, D1: fraction of gradient at ~30 % B, D4: fraction of gradient at ~50 % B, D8: elution at 100 % B. A buffer: 20 mM Tris-HCl, 200 mM NaCl, pH 8.0; B buffer: 20 mM Tris-HCl, 200 mM NaCl, 300 mM imidazole, pH 8.0. Arrow: purified Aerolysin protein, MW: 55.2 kDa. 15 kDa and 10 kDa markers are not resolved.

Sequence and Features