Difference between revisions of "Part:BBa K4497010"

 
 
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<partinfo>BBa_K4497010 short</partinfo>
 
<partinfo>BBa_K4497010 short</partinfo>
  
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This part encodes the intracellular transcription factor function of the MESA system. We used it in combination with mCherry and GFP nanobodies (see [[Part:BBa_K4497017]]).
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==Design & Cloning==
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===Design===
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This part is made of the following components:
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* CD 28 transmembrane domain ([[Part:BBa_K4497004]]): transmembrane anchor for the MESA system
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* TEV Protease Recognition Sequence (M) ([[Part:BBa_K4497006]]): Sequence cut by TEV protease
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* Tetracycline-Induced Transactivator tTA ([[Part:BBa_K4497007]]): Transcription factor of our MESA system
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* EBFP2 ([[Part:BBa_K511100]]): BFP reporter fused to tTA allowing measuring expression and correction of other linked measurements to MESA surface expression.
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===Cloning===
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We did not clone this part individually, it is part of our composite MESA Receptors:
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*G1TA: [[Part:BBa_K4497017]]
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*M1TA: [[Part:BBa_K4497019]]
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*G2TA: [[Part:BBa_K4497021]]
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*M2TA: [[Part:BBa_K4497023]]
  
 
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Latest revision as of 14:07, 13 October 2022


tTA Signaling MESA Part

This part encodes the intracellular transcription factor function of the MESA system. We used it in combination with mCherry and GFP nanobodies (see Part:BBa_K4497017).

Design & Cloning

Design

This part is made of the following components:

  • CD 28 transmembrane domain (Part:BBa_K4497004): transmembrane anchor for the MESA system
  • TEV Protease Recognition Sequence (M) (Part:BBa_K4497006): Sequence cut by TEV protease
  • Tetracycline-Induced Transactivator tTA (Part:BBa_K4497007): Transcription factor of our MESA system
  • EBFP2 (Part:BBa_K511100): BFP reporter fused to tTA allowing measuring expression and correction of other linked measurements to MESA surface expression.

Cloning

We did not clone this part individually, it is part of our composite MESA Receptors:

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 88
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 88
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 88
  • 1000
    COMPATIBLE WITH RFC[1000]