Reporter

Part:BBa_K511100

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)

EBFP2 MammoBlock

An engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces blue. Although this protein has enhanced fluorescence compared to previous blue fluorescent proteins, it still fluoresces significantly more weakly than other common reporters such as EYFP or mKate.

Figures 1 and 2 show typical levels of EBFP2-mediated fluorescence following transfection of HEK-293 cells with EBFP2 driven by the Hef1a low-level constitutive promoter.

For more information, consult the MIT iGEM 2011 wiki here.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aEBFP2 MammoBlock