Difference between revisions of "Part:BBa K4497010"

Revision as of 14:02, 13 October 2022

tTA Signaling MESA Part

This part is made of the following components:

CD 28 transmembrane domain (Part:BBa_K4497004): transmembrane anchor for the MESA system
TEV Protease Recognition Sequence (M) (Part:BBa_K4497006): Sequence cut by TEV protease
Tetracycline-Induced Transactivator tTA (Part:BBa_K4497007): Transcription factor of our MESA system
EBFP2 (Part:BBa_K511100): BFP reporter fused to tTA allowing measuring expression and correction of other linked measurements to MESA surface expression.

We did not clone this part individually, it is part of our composite MESA Receptors:

Sequence and Features

Assembly Compatibility:

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 <partinfo>BBa_K4497010 short</partinfo>
-v
+==Design & Cloning==
+===Design===
+This part is made of the following components:
+* CD 28 transmembrane domain ([[Part:BBa_K4497004]]): transmembrane anchor for the MESA system
+* TEV Protease Recognition Sequence (M) ([[Part:BBa_K4497006]]): Sequence cut by TEV protease
+* Tetracycline-Induced Transactivator tTA ([[Part:BBa_K4497007]]): Transcription factor of our MESA system
+* EBFP2 ([[Part:BBa_K511100]]): BFP reporter fused to tTA allowing measuring expression and correction of other linked measurements to MESA surface expression.
+===Cloning===
+We did not clone this part individually, it is part of our composite MESA Receptors:
+*G1TA: [[Part:BBa_K4497017]]
+*M1TA: [[Part:BBa_K4497019]]
+*G2TA: [[Part:BBa_K4497021]]
+*M2TA: [[Part:BBa_K4497023]]
 <!-- Add more about the biology of this part here