Difference between revisions of "Part:BBa K4438907"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | crp_trigger_4_5’trigger_phi29 (<partinfo>BBa_K4438907</partinfo>) | + | The part crp_trigger_4_5’trigger_phi29 (<partinfo>BBa_K4438907</partinfo>) has complete complementarity to the part Aptamer_crp (<partinfo>BBa_K4438900</partinfo>) and blocks the 5’ region of the CRP aptamer. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. CRP binds with Aptamer_crp (<partinfo>BBa_K4438900</partinfo>) and displaces the crp_trigger_4_5’trigger_phi29 (<partinfo>BBa_K4438907</partinfo>) [1]. This part has complete complementarity with part crp_Target_4(<partinfo>BBa_K4438908</partinfo>). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. |
| + | Different levels of CRP can be detected using all these three parts. | ||
| + | |||
[[File:T--IISER-Tirupati_India--hsa-crp-4.png]] | [[File:T--IISER-Tirupati_India--hsa-crp-4.png]] | ||
Latest revision as of 14:09, 12 October 2022
crp_trigger_4_5trigger_phi29
crp_trigger_4_5’trigger_phi29 (BBa_K4438907) is a single-stranded DNA having 11 nucleotides. Figure 1A) shows the secondary structure and minimum free energy.
Usage and Biology
The part crp_trigger_4_5’trigger_phi29 (BBa_K4438907) has complete complementarity to the part Aptamer_crp (BBa_K4438900) and blocks the 5’ region of the CRP aptamer. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. CRP binds with Aptamer_crp (BBa_K4438900) and displaces the crp_trigger_4_5’trigger_phi29 (BBa_K4438907) [1]. This part has complete complementarity with part crp_Target_4(BBa_K4438908). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of CRP can be detected using all these three parts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967.