Difference between revisions of "Part:BBa K4175012"
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<partinfo>BBa_K4175012 short</partinfo> | <partinfo>BBa_K4175012 short</partinfo> | ||
| − | This is a second-generation anti-CD19 chimeric antigen receptor (CAR) that comprises of a 4-1BB motif (<partinfo>BBa_K4175006</partinfo>) and a CD3ζ motif (<partinfo>BBa_K4175009</partinfo>). It is controlled by UAS-pSV40 promoter, where UAS acts as a binding region for Gal4 while SV40 is a strong promoter. The anti-CD19 CAR is linked to the fluorescent protein, mCherry, by P2A self-cleavage peptide. This peptide will undergo ribosomal skipping upon translation. | + | This is a second-generation anti-CD19 chimeric antigen receptor (CAR) that comprises of a 4-1BB motif (<partinfo>BBa_K4175006</partinfo>) and a CD3ζ motif (<partinfo>BBa_K4175009</partinfo>). It is controlled by UAS-pSV40 promoter (<partinfo>BBa_K4040033</partinfo>), where UAS acts as a binding region for Gal4 while SV40 is a strong promoter. The anti-CD19 CAR is linked to the fluorescent protein, mCherry, by P2A self-cleavage peptide. This peptide will undergo ribosomal skipping upon translation. |
===Sequence and Features=== | ===Sequence and Features=== | ||
Latest revision as of 16:12, 10 October 2022
UAS-pSV40-CAR19-P2A-mCherry
This is a second-generation anti-CD19 chimeric antigen receptor (CAR) that comprises of a 4-1BB motif (BBa_K4175006) and a CD3ζ motif (BBa_K4175009). It is controlled by UAS-pSV40 promoter (BBa_K4040033), where UAS acts as a binding region for Gal4 while SV40 is a strong promoter. The anti-CD19 CAR is linked to the fluorescent protein, mCherry, by P2A self-cleavage peptide. This peptide will undergo ribosomal skipping upon translation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1388
Illegal NgoMIV site found at 1803 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 949