Difference between revisions of "Part:BBa K4175012"

 
 
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<partinfo>BBa_K4175012 short</partinfo>
 
<partinfo>BBa_K4175012 short</partinfo>
  
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This is a second-generation anti-CD19 chimeric antigen receptor (CAR) that comprises of a 4-1BB motif (<partinfo>BBa_K4175006</partinfo>) and a CD3ζ motif (<partinfo>BBa_K4175009</partinfo>). It is controlled by UAS-pSV40 promoter (<partinfo>BBa_K4040033</partinfo>), where UAS acts as a binding region for Gal4 while SV40 is a strong promoter. The anti-CD19 CAR is linked to the fluorescent protein, mCherry, by P2A self-cleavage peptide. This peptide will undergo ribosomal skipping upon translation.  
  
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===Sequence and Features===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4175012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4175012 SequenceAndFeatures</partinfo>
  

Latest revision as of 16:12, 10 October 2022


UAS-pSV40-CAR19-P2A-mCherry

This is a second-generation anti-CD19 chimeric antigen receptor (CAR) that comprises of a 4-1BB motif (BBa_K4175006) and a CD3ζ motif (BBa_K4175009). It is controlled by UAS-pSV40 promoter (BBa_K4040033), where UAS acts as a binding region for Gal4 while SV40 is a strong promoter. The anti-CD19 CAR is linked to the fluorescent protein, mCherry, by P2A self-cleavage peptide. This peptide will undergo ribosomal skipping upon translation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1388
    Illegal NgoMIV site found at 1803
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 949