Difference between revisions of "Part:BBa K4491004"
 
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<partinfo>BBa_K4491004 short</partinfo>
 
<partinfo>BBa_K4491004 short</partinfo>
 
* ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)
 
* ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)
 +
  
 
* Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.  
 
* Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.  
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<html>
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</p>
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</html>
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__TOC__
  
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
−
! colspan="2" style="background:#FFBF00;"|E-cadherin (Preproprotein, Mus Musculus)
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! colspan="2" style="background:#FFBF00;"|DT54
 
|-
 
|-
 
|'''Function'''
 
|'''Function'''
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|Cell-Cell Adhesion
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|Termination
 
|-
 
|-
 
|'''Use in'''
 
|'''Use in'''
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|Mammalian cells
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|Bacteria
 
|-
 
|-
 
|'''Chassis Tested'''
 
|'''Chassis Tested'''
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|Chinese Hamster Ovary (CHO)
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|E. coli
 
|-
 
|-
 
|'''Abstraction Hierarchy'''
 
|'''Abstraction Hierarchy'''
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|-
 
|-
 
|'''Related Device'''
 
|'''Related Device'''
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|[https://parts.igem.org/Part:BBa_K2332313 BBa_K2332313]
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|-
 
|-
 
|'''RFC standard'''
 
|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC10] & [https://parts.igem.org/Help:Assembly_standard_23 RFC23] compatible  
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC10] & [https://parts.igem.org/Help:Assembly_standard_12 RFC12] & [https://parts.igem.org/Help:Assembly_standard_21 RFC21] & [https://parts.igem.org/Help:Assembly_standard_23 RFC23] & [https://parts.igem.org/Help:Assembly_standard_25 RFC25] & [https://parts.igem.org/Help:Assembly_standard_1000 RFC1000] compatible  
 
|-
 
|-
 
|'''Backbone'''
 
|'''Backbone'''
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|pSB1C3<br>  
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|pJUMP18-Uac<br>  
 
|-
 
|-
 
|'''Submitted by'''
 
|'''Submitted by'''
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|[http://2017.igem.org/Team:UCL UCL iGEM 2017]
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|[https://2022.igem.wiki/cambridge/index.html Cambridge iGEM 2022]
 
|}
 
|}
  
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<html>
 
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</p>
 
−
</html>
 
−
__TOC__
 
  
 
'''Secondary Structure'''
 
'''Secondary Structure'''
  
 
[[Image:Mfold-K4491004-1.png]]
 
[[Image:Mfold-K4491004-1.png]]
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===Usage and Biology===
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===DNA Sequence===
  
 
<hr>
 
<hr>
 
'''Measurement'''
 
'''Measurement'''
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Latest revision as of 13:57, 10 October 2022


Terminator.png

DT54

  • ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)


  • Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.

Results characterised by Park et al., 2018

  • Terminator strength (forward): 1800
  • Terminator strength (reverse): 30


DT54
Function Termination
Use in Bacteria
Chassis Tested E. coli
Abstraction Hierarchy Part
Related Device
RFC standard RFC10 & RFC12 & RFC21 & RFC23 & RFC25 & RFC1000 compatible
Backbone pJUMP18-Uac
Submitted by Cambridge iGEM 2022


Secondary Structure

File:Mfold-K4491004-1.png


Usage and Biology

DNA Sequence

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]