Difference between revisions of "Part:BBa K4491004"
 
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| width=50% valign='top' style='border: 1px solid black'|
 
| width=50% valign='top' style='border: 1px solid black'|
 
<partinfo>BBa_K4491004 short</partinfo>
 
<partinfo>BBa_K4491004 short</partinfo>
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* ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.  
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* ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)
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 +
 
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* Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.  
  
 
Results characterised by Park et al., 2018
 
Results characterised by Park et al., 2018
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Terminator strength (forward): 1800
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* Terminator strength (forward): 1800
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Terminator strength (reverse): 30
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* Terminator strength (reverse): 30
  
 
|}
 
|}
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<html>
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</p>
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</html>
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__TOC__
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|DT54
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|-
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|'''Function'''
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|Termination
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|-
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|'''Use in'''
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|Bacteria
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|-
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|'''Chassis Tested'''
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|E. coli
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|-
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|'''Abstraction Hierarchy'''
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|Part
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|-
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|'''Related Device'''
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC10] & [https://parts.igem.org/Help:Assembly_standard_12 RFC12] & [https://parts.igem.org/Help:Assembly_standard_21 RFC21] & [https://parts.igem.org/Help:Assembly_standard_23 RFC23] & [https://parts.igem.org/Help:Assembly_standard_25 RFC25] & [https://parts.igem.org/Help:Assembly_standard_1000 RFC1000] compatible
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|-
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|'''Backbone'''
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|pJUMP18-Uac<br>
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|-
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|'''Submitted by'''
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|[https://2022.igem.wiki/cambridge/index.html Cambridge iGEM 2022]
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|}
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'''Secondary Structure'''
 
'''Secondary Structure'''
  
 
[[Image:Mfold-K4491004-1.png]]
 
[[Image:Mfold-K4491004-1.png]]
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===Usage and Biology===
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===DNA Sequence===
  
 
<hr>
 
<hr>
 
'''Measurement'''
 
'''Measurement'''
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Latest revision as of 13:57, 10 October 2022


Terminator.png

DT54

  • ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)


  • Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.

Results characterised by Park et al., 2018

  • Terminator strength (forward): 1800
  • Terminator strength (reverse): 30


DT54
Function Termination
Use in Bacteria
Chassis Tested E. coli
Abstraction Hierarchy Part
Related Device
RFC standard RFC10 & RFC12 & RFC21 & RFC23 & RFC25 & RFC1000 compatible
Backbone pJUMP18-Uac
Submitted by Cambridge iGEM 2022


Secondary Structure

File:Mfold-K4491004-1.png


Usage and Biology

DNA Sequence

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]