Difference between revisions of "Part:BBa K4491004"

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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
! colspan="2" style="background:#FFBF00;"|DT54
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! colspan="2" style="background:#FFBF00;"|DT54|
 
|'''Function'''
 
|'''Function'''
 
|Termination
 
|Termination

Revision as of 13:56, 10 October 2022


Terminator.png

DT54

  • ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)


  • Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.

Results characterised by Park et al., 2018

  • Terminator strength (forward): 1800
  • Terminator strength (reverse): 30


DT54| Function Termination
Use in Bacteria
Chassis Tested E. coli
Abstraction Hierarchy Part
Related Device
RFC standard RFC10 & RFC12 & RFC21 & RFC23 & RFC25 & RFC1000 compatible
Backbone pJUMP18-Uac
Submitted by Cambridge iGEM 2022


Secondary Structure

File:Mfold-K4491004-1.png


Usage and Biology

DNA Sequence


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]