Difference between revisions of "Part:BBa K4491004"
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* Terminator strength (reverse): 30
 
* Terminator strength (reverse): 30
  
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|}
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|E-cadherin (Preproprotein, Mus Musculus)
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|-
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|'''Function'''
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|Cell-Cell Adhesion
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''Chassis Tested'''
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|Chinese Hamster Ovary (CHO)
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|-
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|'''Abstraction Hierarchy'''
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|Part
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|-
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|'''Related Device'''
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|[https://parts.igem.org/Part:BBa_K2332313 BBa_K2332313]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC10] & [https://parts.igem.org/Help:Assembly_standard_23 RFC23] compatible
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2017.igem.org/Team:UCL UCL iGEM 2017]
 
|}
 
|}
  

Revision as of 11:11, 10 October 2022


Terminator.png

DT54

  • ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)
  • Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.

Results characterised by Park et al., 2018

  • Terminator strength (forward): 1800
  • Terminator strength (reverse): 30
E-cadherin (Preproprotein, Mus Musculus)
Function Cell-Cell Adhesion
Use in Mammalian cells
Chassis Tested Chinese Hamster Ovary (CHO)
Abstraction Hierarchy Part
Related Device BBa_K2332313
RFC standard RFC10 & RFC23 compatible
Backbone pSB1C3
Submitted by [http://2017.igem.org/Team:UCL UCL iGEM 2017]

Secondary Structure

File:Mfold-K4491004-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]