Difference between revisions of "Part:BBa K4214000"
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This part codes for human Gastric Intrinsic Factor protein (AA19-417). The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia. | This part codes for human Gastric Intrinsic Factor protein (AA19-417). The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia. | ||
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| + | [[Image:Part-BBa K4214000.png|450px|thumb|right|Fig.: ''Human GIF (BBa_K4214000 '']] | ||
We '''removed''' the native signal peptide (AA1-18) from the N-terminal in order to obtain a sequence that can be worked with more easily in bacterial systems. However, since we used bacterial and mammalian expression vectors in parallel, we needed to have compatible parts and decided to work only with '''part BBa_K4214001'''[https://parts.igem.org/Part:BBa_K4214001], which '''includes''' the signal peptide sequence (AA1-18). | We '''removed''' the native signal peptide (AA1-18) from the N-terminal in order to obtain a sequence that can be worked with more easily in bacterial systems. However, since we used bacterial and mammalian expression vectors in parallel, we needed to have compatible parts and decided to work only with '''part BBa_K4214001'''[https://parts.igem.org/Part:BBa_K4214001], which '''includes''' the signal peptide sequence (AA1-18). | ||
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==Usage and Biology== | ==Usage and Biology== | ||
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*This part of hGIF should be mentioned that does not contain the signal peptide | *This part of hGIF should be mentioned that does not contain the signal peptide | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4214000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4214000 SequenceAndFeatures</partinfo> | ||
Revision as of 09:13, 9 October 2022
Human Gastric Intrinsic Factor (hGIF)
This part codes for human Gastric Intrinsic Factor protein (AA19-417). The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.
We removed the native signal peptide (AA1-18) from the N-terminal in order to obtain a sequence that can be worked with more easily in bacterial systems. However, since we used bacterial and mammalian expression vectors in parallel, we needed to have compatible parts and decided to work only with part BBa_K4214001[1], which includes the signal peptide sequence (AA1-18).
Usage and Biology
- NCBI page of the human gastric intrinsic factor [2]
- We designed this biobrick in order to have a sequence for our other parts
- This part of hGIF should be mentioned that does not contain the signal peptide
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 265
Illegal BsaI.rc site found at 945
Illegal SapI site found at 935