Difference between revisions of "Part:BBa K4214000"
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*NCBI page of the human gastric intrinsic factor [https://www.ncbi.nlm.nih.gov/gene/2694]
 
*NCBI page of the human gastric intrinsic factor [https://www.ncbi.nlm.nih.gov/gene/2694]
 
*We designed this biobrick in order to use this coding region for generating our mutants so that we ensure the evading of the autoantibodies of pernicious anemia  
 
*We designed this biobrick in order to use this coding region for generating our mutants so that we ensure the evading of the autoantibodies of pernicious anemia  
−
*We used this part with some extra modifications to perform protein surface display in mammalian cells but also protein production in E.coli
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*We used this part with some extra modifications to perform protein surface display in mammalian cells but also protein production in E.coli. These parts are BBa_K4214001 [https://parts.igem.org/Part:BBa_K4214001] , BBa_K4214002 [https://parts.igem.org/Part:BBa_K4214002], BBa_K4214003 [https://parts.igem.org/Part:BBa_K4214003], BBa_K4214004 [https://parts.igem.org/Part:BBa_K4214004].
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*This new part can be found here. (link for new part )
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*This part of hGIF should be mentioned that does not contain the signal peptide
 +
 
  
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4214000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4214000 SequenceAndFeatures</partinfo>

Revision as of 07:35, 9 October 2022

Human Gastric Intrinsic Factor (hGIF)


This part codes for human Gastric Intrinsic Factor. We used the human sequence of the gene. The gene is located in chromosome 11q12.1. This gene is a part of the cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.


Usage and Biology

  • NCBI page of the human gastric intrinsic factor [1]
  • We designed this biobrick in order to use this coding region for generating our mutants so that we ensure the evading of the autoantibodies of pernicious anemia
  • We used this part with some extra modifications to perform protein surface display in mammalian cells but also protein production in E.coli. These parts are BBa_K4214001 [2] , BBa_K4214002 [3], BBa_K4214003 [4], BBa_K4214004 [5].
  • This part of hGIF should be mentioned that does not contain the signal peptide


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 265
    Illegal BsaI.rc site found at 945
    Illegal SapI site found at 935