Difference between revisions of "Part:BBa K4197014"
 
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<partinfo>BBa_K4197014 short</partinfo>
 
<partinfo>BBa_K4197014 short</partinfo>
  
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Constitutive promoter of <i>E. coli</i>.  
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Constitutive promoter for <i>E. coli</i>.  
  
 
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<html>
  
 
<h2>Introduction</h2>
 
<h2>Introduction</h2>
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<p>This part is composed of the gene coding for the 800 first bp of the ihfB promoter. This promoter has been identified as a constitutive <i>E. coli</i> promoter (Weglenska et al., 1996). It is often used by researchers of the Toulouse Biotechnology Institute to express recombinant fluorescent proteins in <i>E. coli</i> (Barthe et al., 2020), as it is strong enough to allow correct expression and weak enough to avoid inclusion bodies.</p>
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<p>This part is composed of the gene coding for the 800 first base pairs of the <i>ihfB</i> promoter. This promoter has been identified as a constitutive <i>E. coli</i> promoter (Weglenska et al., 1996). It was used for constitutive expression of fluorescent proteins in <i>E. coli</i> (Barthe et al., 2020) and appears as strong enough to permit sufficient expression without inclusion bodies.</p>
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<h2>Construction</h2>
 
<h2>Construction</h2>
  
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<p>The objective of the INSA-UPS 2022 team was to use ihfB800 promoter to express mTagBFP (<a href="https://parts.igem.org/Part:BBa_K4197013">BBa_K4197013</a>), mRFP1 (<a href="https://parts.igem.org/Part:BBa_K4197012">BBa K4197012</a>), and mScarlet-I (<a href="https://parts.igem.org/Part:BBa_K4197022">BBa_K4197022</a>) into <i>E. coli</i> Tuner (DE3) cells. The functionality of the promoter was confirmed as the mTagBFP and mScarlet-I were successfully expressed.</p>
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<p>The objective of the INSA-UPS 2022 team was to use the ihfB800 promoter to express mTagBFP (<a href="https://parts.igem.org/Part:BBa_K4197013">BBa_K4197013</a>), mRFP1 (<a href="https://parts.igem.org/Part:BBa_K4197012">BBa_K4197012</a>), and mScarlet-I (<a href="https://parts.igem.org/Part:BBa_K4197022">BBa_K4197022</a>) into <i>E. coli</i> Tuner (DE3) cells. The functionality of the promoter was confirmed as the mTagBFP and mScarlet-I were successfully expressed (see <a href="https://parts.igem.org/Part:BBa_K4197001">BBa_K4197001</a> and <a href="https://parts.igem.org/Part:BBa_K4197021">BBa_K4197021</a> respectively).</p>
 
                                                
 
                                                
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<h2>Conclusion</h2>
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<p>We found that this promoter is very efficient for constitutive expression in <i>E. coli</i>. Do not hesitate to use it for your experimentations.</p>
  
 
<h2>References</h2>
 
<h2>References</h2>
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<p>More information about the project for which the part was created:<a href="https://2022.igem.wiki/toulouse-insa-ups/index.html"> DAISY (INSA-UPS 2022)</a> </p>
 
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Latest revision as of 17:44, 8 October 2022


ihfB800 promoter

Constitutive promoter for E. coli.

Introduction

This part is composed of the gene coding for the 800 first base pairs of the ihfB promoter. This promoter has been identified as a constitutive E. coli promoter (Weglenska et al., 1996). It was used for constitutive expression of fluorescent proteins in E. coli (Barthe et al., 2020) and appears as strong enough to permit sufficient expression without inclusion bodies.

Construction

The objective of the INSA-UPS 2022 team was to use the ihfB800 promoter to express mTagBFP (BBa_K4197013), mRFP1 (BBa_K4197012), and mScarlet-I (BBa_K4197022) into E. coli Tuner (DE3) cells. The functionality of the promoter was confirmed as the mTagBFP and mScarlet-I were successfully expressed (see BBa_K4197001 and BBa_K4197021 respectively).

Conclusion

We found that this promoter is very efficient for constitutive expression in E. coli. Do not hesitate to use it for your experimentations.

References

More information about the project for which the part was created: DAISY (INSA-UPS 2022)

  1. Wȩgleńska, A., Jacob, B., & Sirko, A. (1996). Transcriptional pattern of Escherichia coli ihfB (himD) gene expression. Gene, 181(1-2), 85–88. https://doi.org/10.1016/s0378-1119(96)00468-4
  2. Barthe, M., Tchouanti, J., Gomes, P. H., Bideaux, C., Lestrade, D., Graham, C., Steyer, J.-P., Meleard, S., Harmand, J., Gorret, N., Cocaign-Bousquet, M., & Enjalbert, B. (2020). Availability of the Molecular Switch XylR Controls Phenotypic Heterogeneity and Lag Duration during Escherichia coli Adaptation from Glucose to Xylose. mBio, 11(6), Article e02938-20. https://doi.org/10.1128/mbio.02938-20

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
    Illegal AgeI site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]