Difference between revisions of "Part:BBa K4244007"
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<partinfo>BBa_K4244007 short</partinfo>
 
<partinfo>BBa_K4244007 short</partinfo>
  
−
A silent mutation was performed to removed the BsaI site present in BBa_E0040 at position 664 (g to a) to make it RFC[1000] compatible.
+
Silent mutations were performed to removed the BsaI site at position 664 (G to A), the NheI site at position 6 (T to A) and both AgeI sites at position 32 (C to G) and position 156 (C to G) present in <partinfo>BBa_E0040</partinfo> to make it compatible with all assembly type.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:25, 8 October 2022


GFP without BsaI

Silent mutations were performed to removed the BsaI site at position 664 (G to A), the NheI site at position 6 (T to A) and both AgeI sites at position 32 (C to G) and position 156 (C to G) present in BBa_E0040 to make it compatible with all assembly type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]