Difference between revisions of "Part:BBa T2028"
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This is a chitin binding domain (CBD) designed to be at between protein domains. Chitin binding domains are a type of affinity tag that can be used to purify proteins. | This is a chitin binding domain (CBD) designed to be at between protein domains. Chitin binding domains are a type of affinity tag that can be used to purify proteins. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The chitin binding domain tag is used for protein purification using a chitin column. CBD can be placed on either the N- or C-terminus of proteins. Purification of a CBD-tagged protein is a one-step purification process that yields very pure protein with low miligram yields. The primary disadvantage is that the purification must be done in the absence of thiol-containing reducing agents until the elution step. However, the matrix is compatible with high salt and nonionic detergents. | ||
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Revision as of 18:15, 17 September 2009
Chitin binding internal domain
This is a chitin binding domain (CBD) designed to be at between protein domains. Chitin binding domains are a type of affinity tag that can be used to purify proteins.
Usage and Biology
The chitin binding domain tag is used for protein purification using a chitin column. CBD can be placed on either the N- or C-terminus of proteins. Purification of a CBD-tagged protein is a one-step purification process that yields very pure protein with low miligram yields. The primary disadvantage is that the purification must be done in the absence of thiol-containing reducing agents until the elution step. However, the matrix is compatible with high salt and nonionic detergents.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]