Difference between revisions of "Part:BBa K4368003"
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''BglX'' encodes for the β-glucosidase gene of ''Escherichia coli'' (<partinfo>BBa_K4368002</partinfo>). This enzyme is responsible of the degradation of cellulose working coordinated with th genes cenA and cex. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by glucose concentration (<partinfo>BBa_K118011</partinfo>).
 
''BglX'' encodes for the β-glucosidase gene of ''Escherichia coli'' (<partinfo>BBa_K4368002</partinfo>). This enzyme is responsible of the degradation of cellulose working coordinated with th genes cenA and cex. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by glucose concentration (<partinfo>BBa_K118011</partinfo>).
 
The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
 
The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
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==Characterization==
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xxx
  
 
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Revision as of 14:50, 6 October 2022


pcstA + rbs + bglX + terminator

Description

BglX encodes for the β-glucosidase gene of Escherichia coli (BBa_K4368002). This enzyme is responsible of the degradation of cellulose working coordinated with th genes cenA and cex. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by glucose concentration (BBa_K118011). The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Characterization

xxx

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1609
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1457
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1367

Contribution

  • Group: [1]
  • Author: Molina Calvo, Alonso