Difference between revisions of "Part:BBa K4368003"

Revision as of 10:25, 6 October 2022

pcstA + rbs + bglX + terminator

Description

BglX encodes for the β-glucosidase gene of Escherichia coli (BBa_K4368002). This enzyme is responsible of the degradation of celullose working coordinated with th genes cenA and cex. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by glucose concentration (BBa_K118011). The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1609
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1457
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1367

Contribution

Group: [1]
Author: Molina Calvo, Alonso

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4368003 short</partinfo>
+==Description ==
 ''BglX'' encodes for the β-glucosidase gene of ''Escherichia coli'' (<partinfo>BBa_K4368002</partinfo>). This enzyme is responsible of the degradation of celullose working coordinated with th genes cenA and cex. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by glucose concentration (<partinfo>BBa_K118011</partinfo>).
 The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.