Difference between revisions of "Part:BBa K4115044"
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==Proof of function== | ==Proof of function== | ||
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We transformed this plasmid into E. coli and encapsulated the E. coli into alginate gel beads. It was then induced to express tagRFP. We photograph the cross section of the gel beads under a confocal microscope and get Figure 1. | We transformed this plasmid into E. coli and encapsulated the E. coli into alginate gel beads. It was then induced to express tagRFP. We photograph the cross section of the gel beads under a confocal microscope and get Figure 1. | ||
[[File:TagRFP in gel beads.jpg|700px|]] | [[File:TagRFP in gel beads.jpg|700px|]] | ||
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'''Figure 1:''' Confocal image of cross-section of gel beads with red fluorescent protein E.coli. | '''Figure 1:''' Confocal image of cross-section of gel beads with red fluorescent protein E.coli. | ||
Revision as of 06:58, 3 October 2022
J23101-B0034-tagRFP-B0015
| CMV:Ha-NLS-dCas9-Linker-VP16-NLS:BGH | |
|---|---|
| Function | gene reporter |
| Use in | E.coli |
| RFC standard | RFC 10, RFC 10 compatible |
| Backbone | pBBR1 |
| Submitted by | (ShanghaiTech_China) |
This composite part is used to express the tagRFP(BBa_K4115013) by a constitutive promoter J23101(J23101), thus we can use red fluorescence as a detection.
Proof of function
We transformed this plasmid into E. coli and encapsulated the E. coli into alginate gel beads. It was then induced to express tagRFP. We photograph the cross section of the gel beads under a confocal microscope and get Figure 1.
Figure 1: Confocal image of cross-section of gel beads with red fluorescent protein E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 695
Illegal SapI.rc site found at 77