Difference between revisions of "Part:BBa K4115044"

Revision as of 06:15, 3 October 2022

J23101-B0034-tagRFP-B0015

CMV:Ha-NLS-dCas9-Linker-VP16-NLS:BGH
Function	gene reporter
Use in	E.coli
RFC standard	RFC 10, RFC 10 compatible
Backbone	pBBR1
Submitted by	[1]

This composite part is used to express the tagRFP(BBa_K4115013) by a constitutive promoter J23101(J23101), thus we use red fluorescence as a detection.

Proof of function

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 695
Illegal SapI.rc site found at 77

@@ Line 9: / Line 9: @@
 |-
 |'''Use in'''
-|Prokaryotic cells
+|E.coli
 |-
 |'''RFC standard'''
@@ Line 18: / Line 18: @@
 |-
 |'''Submitted by'''
-|[http://2022.igem.org/Team:ShanghaiTech_China]
+|[https://2022.igem.wiki/shanghaitech-china/]
 |}