Difference between revisions of "Part:BBa T2016:Design"
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
This head domain begins with Met-Lys to promote efficient translation of the protein in ''E. coli''.
+
 
 +
This head domain begins with Met-Lys to promote efficient translation of the protein in ''E. coli''.  Rare codons from various model organisms were avoided where possible.
  
 
===Source===
 
===Source===

Revision as of 13:11, 3 September 2009

S-tag head domain (KETAAAKFERQHMDS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 16
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 10


Design Notes

This head domain begins with Met-Lys to promote efficient translation of the protein in E. coli. Rare codons from various model organisms were avoided where possible.

Source

This is a synthetic sequence

References

<biblio>

  1. Kim93 pmid=8453373

// appears to be original reference

  1. Kim94 pmid=8059949
  2. Fritze pmid=11044970

</biblio>