Difference between revisions of "Part:BBa K4226005"
 
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<partinfo>BBa_K4226005 short</partinfo>
 
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[[Part:BBa K4226000|3WJ-Bro]] and [[Part:BBa_K3977002|mScarlet-I]] were used as RNA and protein reporting systems.  
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[[Part:BBa K4226000|3WJ-Bro]] and [[Part:BBa_K3977002|mScarlet-I]] together were used as RNA and protein reporting systems.  
 
3WJ-Bro can be applied as a gene marker in creating a system for reporting the presence and expression of target genes. mScarlet-I is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)
 
3WJ-Bro can be applied as a gene marker in creating a system for reporting the presence and expression of target genes. mScarlet-I is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)
  
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<partinfo>BBa_K4226005 parameters</partinfo>
 
<partinfo>BBa_K4226005 parameters</partinfo>
 
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<b>Reference</b>
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<br>
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[1] Bai, Jiuyuan, et al. "A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering." Nature communications 11.1 (2020): 1-14.<br>
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[2] Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for cellular imaging." Nature methods 14.1 (2017): 53-56.<br>

Latest revision as of 05:55, 28 September 2022


RNA and protein reporting systems

3WJ-Bro and mScarlet-I together were used as RNA and protein reporting systems. 3WJ-Bro can be applied as a gene marker in creating a system for reporting the presence and expression of target genes. mScarlet-I is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 780
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference
[1] Bai, Jiuyuan, et al. "A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering." Nature communications 11.1 (2020): 1-14.
[2] Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for cellular imaging." Nature methods 14.1 (2017): 53-56.