Difference between revisions of "Part:BBa K4432221:Design"

 
 
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===Design Notes===
 
===Design Notes===
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to do
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pLac promoter ([[Part:BBa_R0010|BBa_R0010]]) + custom made RBS ([[Part:BBa_K4432011|BBa_K4432011]]) + CrtO coding sequence ([[Part:BBa_K4432021|BBa_K4432021]])
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+
−
 
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===Source===
 
===Source===
  
−
to do
+
DNA synthesis, PCR amplification & Assembly
  
 
===References===
 
===References===

Latest revision as of 21:15, 26 September 2022


CrtO expression cassette under the control of the pLac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 681
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pLac promoter (BBa_R0010) + custom made RBS (BBa_K4432011) + CrtO coding sequence (BBa_K4432021)

Source

DNA synthesis, PCR amplification & Assembly

References