Difference between revisions of "Part:BBa K145150"
 
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Hybrid promoter consisting of the Lux box upstream the -35 box and binding sites for P22 C2; O<sub>R2</sub> and O<sub>R1</sub> located between the -35 and -10 boxes and downstream of them respectively. Transcription from this promoter can be activated by a LuxR-HSL complex and repressed by p22 C2. Background transcription from this pomoter (no activation and no repression) is very low.
 
Hybrid promoter consisting of the Lux box upstream the -35 box and binding sites for P22 C2; O<sub>R2</sub> and O<sub>R1</sub> located between the -35 and -10 boxes and downstream of them respectively. Transcription from this promoter can be activated by a LuxR-HSL complex and repressed by p22 C2. Background transcription from this pomoter (no activation and no repression) is very low.
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Comment
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Sequence read does not match part. Part was made with two primers and Klenow end-filling. These [https://parts.igem.org/Part:BBa_K145150:Design primers] are available on [http://2008.igem.org/Team:KULeuven request].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:15, 22 July 2009

Hybrid promoter: HSL-LuxR activated, P22 C2 repressed

Hybrid promoter consisting of the Lux box upstream the -35 box and binding sites for P22 C2; OR2 and OR1 located between the -35 and -10 boxes and downstream of them respectively. Transcription from this promoter can be activated by a LuxR-HSL complex and repressed by p22 C2. Background transcription from this pomoter (no activation and no repression) is very low.

Comment

Sequence read does not match part. Part was made with two primers and Klenow end-filling. These primers are available on [http://2008.igem.org/Team:KULeuven request].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]