Difference between revisions of "Part:BBa J428343"

Latest revision as of 18:07, 12 July 2022

pJUMP29-1C KanR Type IIS Level 1 vector for C modules

OriV 9 (pBBR322/ROP; medium copy number)

JUMP Collection

Joint Universal Modular Plasmids (JUMP) is a modular vector platform for Golden Gate (PhytoBrick) and Biobrick assembly based on Standard European Vector Architecture (SEVA).

For more information please see: Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003

Plasmid	Name	Origin (Copy)	ABr	Level	Module	Selection	2022 Distribution Location
BBa_J428341	pJUMP29-1A	pBBR322 (Medium)	Kanamycin	Level 1	A	BBa_J428326	Kit Plate 1 Well 2A
BBa_J428342	pJUMP29-1B	pBBR322 (Medium)	Kanamycin	Level 1	B	BBa_J428326	Kit Plate 1 Well 2C
BBa_J428343	pJUMP29-1C	pBBR322 (Medium)	Kanamycin	Level 1	C	BBa_J428326	Kit Plate 1 Well 2E
BBa_J428344	pJUMP29-1D	pBBR322 (Medium)	Kanamycin	Level 1	D	BBa_J428326	Kit Plate 1 Well 2G

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 2948
Illegal suffix found at 16
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2948
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NotI site found at 24
Illegal NotI site found at 2954
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2948
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 2948
Illegal suffix found at 17
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 2948
Plasmid lacks a suffix.
Illegal XbaI site found at 2963
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NgoMIV site found at 1390
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 2188

@@ Line 4: / Line 4: @@
 OriV 9 (pBBR322/ROP; medium copy number)
+===JUMP Collection===
+{{Template:Collection/JUMP/Description}}
+{{Template:Collection/JUMP/Level1/Plasmids}}
 <!-- Add more about the biology of this part here
@@ Line 10: / Line 15: @@
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-Please view and default to the original genbank files from the Supplementary Data in https://doi.org/10.1093/synbio/ysab003 as the Registry sequence is in draft.
 <partinfo>BBa_J428343 SequenceAndFeatures</partinfo>