Difference between revisions of "Part:BBa J428100"

Latest revision as of 00:12, 6 July 2022

Interlab_NegC

Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.

Specially designed for the Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors studies.

It can be used as negative control for fluorescent measurements in general.

Figure 1. SBOL Visual representation of the device J428100 from 2022 Interlab studies.

Interlab 2022 Experiment 1 Device

Negative Control for Experiment 1 of the 2022 iGEM Interlab

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 1.

Interlab 2022 Experiment 2 Device

Negative Control for Experiment 2 of the 2022 iGEM Interlab

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 2.

Interlab 2022 Experiment 3 Device

Negative Control for Experiment 3 of the 2022 iGEM Interlab

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 3.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 71
Illegal NheI site found at 94
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_J428100 short</partinfo>
 Non-fluorescent negative control for InterLab studies 2022.
 It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR).
-It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpb_T1).
+It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1).
 It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.
+Specially designed for the Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors studies.
-Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors
+It can be used as negative control for fluorescent measurements in general.
 [[File:EngCom_2022_J428100.png|500px|thumb|center|alt=J428100.|Figure 1. SBOL Visual representation of the device [https://parts.igem.org/Part:BBa_J428100 J428100] from [https://technology.igem.org/interlabs 2022 Interlab studies.] ]]
-<!-- Add more about the biology of this part here
-===Usage and Biology===
-<!-- -->
+===Interlab 2022 Experiment 1 Device===
+* Negative Control for Experiment 1 of the 2022 iGEM Interlab
+{{Template:Collection/Interlab_2022/Experiment_1/Partof}}
+===Interlab 2022 Experiment 2 Device===
+* Negative Control for Experiment 2 of the 2022 iGEM Interlab
+{{Template:Collection/Interlab_2022/Experiment_2/Partof}}
+===Interlab 2022 Experiment 3 Device===
+* Negative Control for Experiment 3 of the 2022 iGEM Interlab
+{{Template:Collection/Interlab_2022/Experiment_3/Partof}}
+===Usage and Biology===
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_J428100 SequenceAndFeatures</partinfo>