Difference between revisions of "Part:BBa J428100"
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| − | ===Interlab 2022 Experiment 1 | + | ===Interlab 2022 Experiment 1 Device=== |
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Revision as of 17:55, 5 July 2022
Interlab_NegC
Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.
Specially designed for the Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors studies.
It can be used as negative control for fluorescent measurements in general.
Interlab 2022 Experiment 1 Device
The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 1.
Interlab 2022 Experiment 2 Device
The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 2.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 71
Illegal NheI site found at 94 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]