Difference between revisions of "Part:BBa K4032105"
Line 16: Line 16:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
DegP is a type of heat shock protein in E.coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.  
+
DegP is a type of heat shock protein in ''E. coli''. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.  
  
−
It is also a type of serine protease due to the presence of serine residues in the active center.
+
It is also a type of serine protease in terms of the presence of serine residues in the active center.
  
 
In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.
 
In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.
Line 25: Line 25:
 
===Designs===
 
===Designs===
  
−
The lac promoter , the RBS and the double terminator are added to <partinfo>BBa_K4032002</partinfo>, forming this part.
+
The lac promoter, the RBS and the double terminator are added to <partinfo>BBa_K4032002</partinfo>, forming this part.
  
−
The lac promoter , the RBS and the double terminator are used from <partinfo>BBa_J04450</partinfo>.
+
The lac promoter, the RBS and the double terminator are from <partinfo>BBa_J04450</partinfo>.
  
 
This part was created by the In-Fusion method.
 
This part was created by the In-Fusion method.

Revision as of 07:00, 21 October 2021


lacI+RBS+DegP+double terminator

Contents:

・The lac promoter (from BBa_R0010)

・The RBS (from BBa_B0034)

・The gene of DegP protein (from BBa_K4032002)

・The double terminator (from BBa_B0015)


Usage and Biology

DegP is a type of heat shock protein in E. coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.

It is also a type of serine protease in terms of the presence of serine residues in the active center.

In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.


Designs

The lac promoter, the RBS and the double terminator are added to BBa_K4032002, forming this part.

The lac promoter, the RBS and the double terminator are from BBa_J04450.

This part was created by the In-Fusion method.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1364
    Illegal AgeI site found at 2206
    Illegal AgeI site found at 2318
  • 1000
    COMPATIBLE WITH RFC[1000]