Difference between revisions of "Help:Plasmid backbones"

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{{:Plasmids/Overview}}
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[[image:PlasmidDNA.png]]
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded units of DNA that replicate within a cell independent of the chromosomal DNA within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell]. They are often acquired by cells and can bestow the benefit of antibiotic resistance or protein production. Plasmids are used to incorporate biobrick devices and systems within cells via transformation.
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*'''[[Help:Plasmids/Nomenclature | Plasmid Naming]]''' - Information about our naming conventions for BioBrick compatible vectors (like pSB1AC3).
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*'''[[Help:Plasmid backbones/Glossary|Glossary]]''' - What are plasmids, construction plasmids and backbones?
  
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*'''[[Help:Plasmids/Construction Plasmids| Types of Plasmids]]''' - What's a construction plasmid? Does "plasmid" mean vector backbone only?
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*'''[[Help:Plasmid backbones/Features|Plasmid backbone features]]''' - Common features of plasmid backbones
  
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*'''[[Help:Plasmid features|Plasmid features]]''' - Features of plasmid parts & their  feature tables of plasmids mean
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*'''[[Help:Plasmid backbones/Nomenclature|Naming new plasmid backbones]]''' - How BioBrick plasmid backbones are named
  
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*Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid plasmid parts][[Image:Part icon plasmid.png]]
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*'''[[Help:Plasmid backbones/Construction|Constructing new plasmid backbones]]''' - How to construct new BioBrick® plasmid backbones
  
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*'''[[Help:Plasmid backbones/Entering new plasmids in the Registry|Entering new plasmid backbones and their default inserts in the Registry]]''' - How to enter new plasmid backbones in the Registry
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===Antibiotic Resistance===
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By incorporating an antibiotic-resistance gene on a plasmid, engineered-cells are able to be selected for using the appropriate antibiotic. The most common resistances are ampicillin ("Amp"), kanomycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Chlor").  For more information on which plasmids carry these resistances, click the [[Help:Plasmids/Nomenclature |nomenclature]] documentation section.   
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*'''[[Plasmid backbones|Browse available plasmid backbones]]''' [[Image:Part icon plasmid.png]]
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All of our plasmids are available through the use of [[Part:BBa_P1010| BBa_P1010]]. (Information on how to use that part is available on its part page)</b>
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*'''<span style="color:red; font-weight:bold; font-size:115%;">NEW:</span>[[Help:Plasmid_Backbone_Availability_2009|Plasmid Backbone Availability 2009]]'''

Latest revision as of 18:31, 10 June 2009

BioBrickbackbonevspart.png

Plasmids are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the cell independently of the chromosomal DNA. Plasmid DNA is easily purified from cells, manipulated using common lab techniques and incorporated into cells. Most BioBrick parts in the Registry are maintained and propagated on plasmids. Thus, construction of BioBrick parts, devices and systems usually requires working with plasmids.

Note: In the Registry, plasmids are made up of two distinct components:

  1. the BioBrick part, device or system that is located in the BioBrick cloning site, between (and excluding) the BioBrick prefix and suffix.
  2. the plasmid backbone which propagates the BioBrick part. The plasmid backbone is defined as the sequence beginning with the BioBrick suffix, including the replication origin and antibiotic resistance marker, and ending with the BioBrick prefix. [Note that the plasmid backbone itself can be composed of BioBrick parts.]

Many BioBrick parts in the Registry are maintained on more than one plasmid backbone!

  • Glossary - What are plasmids, construction plasmids and backbones?