Difference between revisions of "Part:BBa P1003"
 
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Kanamycin resistance cassette including promoter and coding sequence.  It lacks a terminator.
 
Kanamycin resistance cassette including promoter and coding sequence.  It lacks a terminator.
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===Usage and Biology===
 
===Usage and Biology===
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*Used for [[Help:Plasmids/Construction|modular BioBrick vector construction]] using BioBrick base vector [[Part:BBa_I51020|BBa_I51020]].
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===MiamiU_OH Contribution===
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This part was optimized for expression and function in the photosynthetic chassis Synechococcus elongatus PCC 7942. The promoter can be found at <partinfo>BBa_K4047020</partinfo> and the coding sequence can be found at <partinfo>BBa_K4047019</partinfo>.
  
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Designed for use in modular BioBricks vector construction.
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===References===
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'''Engineering BioBrick vectors from BioBrick parts'''<br>
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''Journal of Biological Engineering,'' 2008 Apr 14;2:5<br>
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Reshma Shetty, Drew Endy, Tom Knight<br>
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[http://www.jbioleng.org/content/2/1/5 URL]<br>
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656101 GenBank EU496093]
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 20:42, 20 October 2021

kanamycin resistance cassette

Kanamycin resistance cassette including promoter and coding sequence. It lacks a terminator.

Usage and Biology

MiamiU_OH Contribution

This part was optimized for expression and function in the photosynthetic chassis Synechococcus elongatus PCC 7942. The promoter can be found at BBa_K4047020 and the coding sequence can be found at BBa_K4047019.

References

Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656101 GenBank EU496093]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]