Difference between revisions of "Part:BBa K3889002"
AshWinShaRma (Talk | contribs) |
AshWinShaRma (Talk | contribs) |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K3889002 short</partinfo> | <partinfo>BBa_K3889002 short</partinfo> | ||
| − | sfGFP is a variant of Green Fluorescence | + | sfGFP is a variant of Green Fluorescence Protein from <i>Aequorea victoria</i> and has the following parameters [1]: |
{| class=wikitable | {| class=wikitable | ||
|- | |- | ||
| Line 27: | Line 27: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | sfGFP can be used to report protein production or in systems where fluorescence is required like FRET. In <partinfo>BBa_K3889102</partinfo>, <partinfo>BBa_K3889110</partinfo> and <partinfo>BBa_K3889122</partinfo> we planned to use sfGFP as a reporter for | + | sfGFP can be used to report protein production or in systems where fluorescence is required like FRET. In <partinfo>BBa_K3889102</partinfo>, <partinfo>BBa_K3889110</partinfo> and <partinfo>BBa_K3889122</partinfo> we planned to use sfGFP as a reporter for ovastacin, xylR and mf-Lon production respectively in <i>Bacillus subtilis</i> 168. In <partinfo>BBa_K3889130</partinfo>, sfGFP along with <partinfo>BBa_J06504</partinfo> for checking terminator efficiency in <i>Bacillus subtilis</i> 168. |
| − | + | ||
| + | This is a modified version of <partinfo>BBa_I746916</partinfo> specific to our project's assembly. Mutated Kpn1 site and changed stop codon to make assembly compatible part. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 16:55, 20 October 2021
modified_sfGFP
sfGFP is a variant of Green Fluorescence Protein from Aequorea victoria and has the following parameters [1]:
| Ex : Peak excitation wavelength (in nm) | 485 |
|---|---|
| Em: Peak emission wavelength (in nm) | 510 |
| EC: Extinction Coefficient (M-1 cm-1) | 83,300 |
| QY: Quantum Yield | 0.65 |
| Brightness | 54.15 |
| Maturation (min) | 13.6 |
Usage and Biology
sfGFP can be used to report protein production or in systems where fluorescence is required like FRET. In BBa_K3889102, BBa_K3889110 and BBa_K3889122 we planned to use sfGFP as a reporter for ovastacin, xylR and mf-Lon production respectively in Bacillus subtilis 168. In BBa_K3889130, sfGFP along with BBa_J06504 for checking terminator efficiency in Bacillus subtilis 168.
This is a modified version of BBa_I746916 specific to our project's assembly. Mutated Kpn1 site and changed stop codon to make assembly compatible part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 13