Difference between revisions of "Part:BBa K3889002"

 
 
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<partinfo>BBa_K3889002 short</partinfo>
 
<partinfo>BBa_K3889002 short</partinfo>
  
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sfGFP is a variant of Green Fluorescence Protein. It can be used for reporting the expression of the desired promoter.
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sfGFP is a variant of Green Fluorescence Protein from <i>Aequorea victoria</i> and has the following parameters [1]:
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{| class=wikitable
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|-
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!Ex : Peak excitation wavelength (in nm)
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|485
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|-
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!Em: Peak emission wavelength (in nm)
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|510
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|-
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!EC: Extinction Coefficient  (M<sup>-1</sup> cm<sup>-1</sup>)
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|83,300
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|-
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!QY: Quantum Yield
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|0.65
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|-
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!Brightness
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|54.15
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|-
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!Maturation (min)
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|13.6
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|}
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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sfGFP can be used to report protein production or in systems where fluorescence is required like FRET. In <partinfo>BBa_K3889102</partinfo>, <partinfo>BBa_K3889110</partinfo> and <partinfo>BBa_K3889122</partinfo> we planned to use sfGFP as a reporter for ovastacin, xylR and mf-Lon production respectively in <i>Bacillus subtilis</i> 168. In <partinfo>BBa_K3889130</partinfo>, sfGFP along with <partinfo>BBa_J06504</partinfo> for checking terminator efficiency in <i>Bacillus subtilis</i> 168.
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This is a modified version of <partinfo>BBa_I746916</partinfo> specific to our project's assembly. Mutated Kpn1 site and changed stop codon to make assembly compatible part.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889002 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3889002 parameters</partinfo>
 
<partinfo>BBa_K3889002 parameters</partinfo>
 
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===References===
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1. https://www.fpbase.org/protein/superfolder-gfp/

Latest revision as of 16:55, 20 October 2021


modified_sfGFP

sfGFP is a variant of Green Fluorescence Protein from Aequorea victoria and has the following parameters [1]:

Ex : Peak excitation wavelength (in nm) 485
Em: Peak emission wavelength (in nm) 510
EC: Extinction Coefficient (M-1 cm-1) 83,300
QY: Quantum Yield 0.65
Brightness 54.15
Maturation (min) 13.6


Usage and Biology

sfGFP can be used to report protein production or in systems where fluorescence is required like FRET. In BBa_K3889102, BBa_K3889110 and BBa_K3889122 we planned to use sfGFP as a reporter for ovastacin, xylR and mf-Lon production respectively in Bacillus subtilis 168. In BBa_K3889130, sfGFP along with BBa_J06504 for checking terminator efficiency in Bacillus subtilis 168.

This is a modified version of BBa_I746916 specific to our project's assembly. Mutated Kpn1 site and changed stop codon to make assembly compatible part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13



References

1. https://www.fpbase.org/protein/superfolder-gfp/