Difference between revisions of "Part:BBa K4032105"

Revision as of 12:14, 20 October 2021

lacI+RBS+DegP+double terminator

Contents:

・The lac promoter (from BBa_R0010)

・The RBS (from BBa_B0034)

・The gene of DegP protein (from BBa_K4032002)

・The double terminator (from BBa_B0015)

DegP is a type of heat shock protein in E.coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.

It is also a type of serine protease due to the presence of serine residues in the active center.

In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.

The lac promoter , the RBS and the double terminator are added to BBa_K4032002, forming this part.

The lac promoter , the RBS and the double terminator are used from BBa_J04450.

This part was created by the In-Fusion method.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1364
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1364
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1364
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1364
Illegal AgeI site found at 2206
Illegal AgeI site found at 2318
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ・The lac promoter (from <partinfo>BBa_R0010</partinfo>)
-・The  RBS (from <partinfo>BBa_B0034<//partinfo>)
+・The  RBS (from <partinfo>BBa_B0034</partinfo>)
-・The gene of DegP protein.
+・The gene of DegP protein (from <partinfo>BBa_K4032002</partinfo>)
 ・The double terminator (from <partinfo>BBa_B0015</partinfo>)
@@ Line 25: / Line 25: @@
 ===Designs===
-The lac promoter , the RBS and the double terminator are added to <partinfo>BBa_K40320xx</partinfo>, forming this part.
+The lac promoter , the RBS and the double terminator are added to <partinfo>BBa_K4032002</partinfo>, forming this part.
 The lac promoter , the RBS and the double terminator are used from <partinfo>BBa_J04450</partinfo>.