Difference between revisions of "Part:BBa K4032101"
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This part expresses the α-galactosidase-RFP fusion protein. | This part expresses the α-galactosidase-RFP fusion protein. | ||
| − | α-galactosidase is from E.coli K-12 MG1655 and RFP is from <partinfo>BBa_J04450/partinfo>. | + | α-galactosidase is from E.coli K-12 MG1655 and RFP is from <partinfo>BBa_J04450/<partinfo>. |
We removed the stop codon of alpha-galactosidase and the start codon of RFP and connected the ends. | We removed the stop codon of alpha-galactosidase and the start codon of RFP and connected the ends. | ||
Revision as of 11:28, 20 October 2021
lacI+RBS+α-gal-RFP+double terminator
Contents :
・The lac promoter (from BBa_R0010)
・The RBS (from BBa_B0034)
・The gene of the α-galactosidase-RFP fusion protein.
・The double terminator (from BBa_B0015)
Usage and Biology
This enzyme catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Red fluorescence of RFP is observed under the fluorescence microscope and natural light.
Design
This part expresses the α-galactosidase-RFP fusion protein.
α-galactosidase is from E.coli K-12 MG1655 and RFP is from No part name specified with partinfo tag.