Difference between revisions of "Part:BBa K1969005"
 
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<partinfo>BBa_K1969005 short</partinfo>
 
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This is the full length of CUP1 promoter in yeast Saccharomyces cerevisiae. As the major copper-activated metallothionine in yeast, Cup1p binds and sequesters cuprous copper(I), Cu+, providing the principal method of removing this metal ion from the cell.  CUP1 transcription is specifically induced by the copper-dependent transcription activator Cup2p (more commonly known as Ace1p) in response to high levels of copper ions and by Hsf1p in response to heat shock, glucose starvation and oxidation stress. In the presence of copper, Cup1p is also capable of antioxidant activity and thus contributes a significant, albeit minor, role to oxygen radical detoxification, especially in the absence of Cu,Zn-superoxide dismutase Sod1p.
 
 
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<h3>Characterization</h3>
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This is the full length of CUP1 promoter in yeast Saccharomyces cerevisiae. As the major copper-activated metallothionine in yeast, Cup1p binds and sequesters cuprous copper(I), Cu+, providing the principal method of removing this metal ion from the cell.  CUP1 transcription is specifically induced by the copper-dependent transcription activator Cup2p (more commonly known as Ace1p) in response to high levels of copper ions and by Hsf1p in response to heat shock, glucose starvation and oxidation stress. In the presence of copper, Cup1p is also capable of antioxidant activity and thus contributes a significant, albeit minor, role to oxygen radical detoxification, especially in the absence of Cu,Zn-superoxide dismutase Sod1p.  
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<p><a href="https://2021.igem.org/Team:Toulouse_INSA-UPS">Toulouse_INSA_UPS_2021</a>contributed to the characterization of this part. The pCUP1 promoter, inducible by the addition of copper in the medium, had not been characterized before, but the team showed this year that it is functional for expression in S. cerevisiae. To do this the promoter was placed upon the <i>dbr1</i> gene, allowing the production of dihydro-&beta;-ionone and this molecule was indentified thanks to GC-MS approaches. Check the part of the full construction <a href="https://parts.igem.org/Part:BBa_K3930000" class="pr-0" target="_blank">(BBa_K3930000)</a> for more result details.</p>
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(<small>--[[User:Cecilia34|Cecilia34]] 10:10, 23 October 2020 (UTC+2)</small>)<br>
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<h2>Contribution from other teams</h2>
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<h3>Toulouse_INSA-UPS 2021's contribution</h3>
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<h4>Characterization</h4>
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<p> <a href="https://2021.igem.org/Team:Toulouse_INSA-UPS" class="pr-0" target="_blank"> Toulouse_INSA_UPS_2021</a>contributed to the characterization of this part. The part BBa_K1969005 for the CUP1 promoter, inducible by the addition of copper in the medium, had not been characterized before in iGEM, but the team showed this year that it is functional for expression in <i>S. cerevisiae</i>. To do this the promoter was placed upon the <i>dbr1</i> gene, allowing the production of dihydro-&beta;-ionone and this molecule was identified thanks to GC-MS approaches. Check the part of the full construction <a href="https://parts.igem.org/Part:BBa_K3930000" class="pr-0" target="_blank">(BBa_K3930000)</a> for more result details.</p>
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(<small>--[[User:ThomasG|ThomasG]] 16:53, 18 October 2021 (UTC+2)</small>)
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 18:32, 19 October 2021


CUP1 promoter This is the full length of CUP1 promoter in yeast Saccharomyces cerevisiae. As the major copper-activated metallothionine in yeast, Cup1p binds and sequesters cuprous copper(I), Cu+, providing the principal method of removing this metal ion from the cell. CUP1 transcription is specifically induced by the copper-dependent transcription activator Cup2p (more commonly known as Ace1p) in response to high levels of copper ions and by Hsf1p in response to heat shock, glucose starvation and oxidation stress. In the presence of copper, Cup1p is also capable of antioxidant activity and thus contributes a significant, albeit minor, role to oxygen radical detoxification, especially in the absence of Cu,Zn-superoxide dismutase Sod1p.

Contribution from other teams


Toulouse_INSA-UPS 2021's contribution


Characterization

Toulouse_INSA_UPS_2021contributed to the characterization of this part. The part BBa_K1969005 for the CUP1 promoter, inducible by the addition of copper in the medium, had not been characterized before in iGEM, but the team showed this year that it is functional for expression in S. cerevisiae. To do this the promoter was placed upon the dbr1 gene, allowing the production of dihydro-β-ionone and this molecule was identified thanks to GC-MS approaches. Check the part of the full construction (BBa_K3930000) for more result details.

(--ThomasG 16:53, 18 October 2021 (UTC+2))

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 64
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]