Difference between revisions of "Part:BBa K3889101"

 
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<partinfo>BBa_K3889101 short</partinfo>
 
<partinfo>BBa_K3889101 short</partinfo>
  
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Composite part for P22 expression and fluorescence check-in bacillus
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<partinfo>BBa_K3889150</partinfo> contains SRTF1 binding site (<partinfo>BBa_K3889030</partinfo>) allowing expression of P22 c2 repressor (<partinfo>BBa_K3889020</partinfo>) only in the presence of progesterone if SRTF1 (<partinfo>BBa_K3889021</partinfo>) is being produced constitutively.
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Azurite was linked with the help of P2A linker (<partinfo>BBa_K3889069</partinfo>)in order to coexpress it with P22 c2 repressor and check protein expression
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
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This part along with <partinfo>BBa_K3889100</partinfo> and <partinfo>BBa_K3889102</partinfo> was used in order to produce Ovastacin (<partinfo>BBa_K3889022</partinfo>) when progesterone in absent in the fallopian tube. The working of the system is shown below:
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<html>
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<body>
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<video style="width: 100%" muted id="myVideo" controls autoplay loop>
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<source src="https://static.igem.org/mediawiki/parts/c/c7/T--IISER-Tirupati_India--ovastacin_production.mov">
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</video>
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</html>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889101 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889101 SequenceAndFeatures</partinfo>

Revision as of 12:19, 19 October 2021


P22 expression cassette Bacillus subtilis

BBa_K3889150 contains SRTF1 binding site (BBa_K3889030) allowing expression of P22 c2 repressor (BBa_K3889020) only in the presence of progesterone if SRTF1 (BBa_K3889021) is being produced constitutively.

Azurite was linked with the help of P2A linker (BBa_K3889069)in order to coexpress it with P22 c2 repressor and check protein expression

Usage and Biology

This part along with BBa_K3889100 and BBa_K3889102 was used in order to produce Ovastacin (BBa_K3889022) when progesterone in absent in the fallopian tube. The working of the system is shown below:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]