Difference between revisions of "Part:BBa K3889100"

Revision as of 12:13, 19 October 2021

SRTF1 expression cassette Bacillus subtilis

SRTF1 is expressed under a constitutive promoter SP126 (BBa_K3889010). In absence of progesterone it can bind to its binding site (BBa_K3889030) and repress transcription of the gene. Now as progesterone enters the system SRTF1 binds to progesterone leaving its binding site free for the transcription machinery to start working. This results in a progesterone inducible gene expression.

mcherry was linked with the help of P2A linker (BBa_K3889069)in order to coexpress it with SRTF1 and check protein expression

Usage and Biology

This part along with BBa_K3889101 and BBa_K3889102 was used in order to produce Ovastacin (BBa_K3889022) when progesterone in absent in the fallopian tube. The working of the system is shown below:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 SRTF1 is expressed under a constitutive promoter SP126 (<partinfo>BBa_K3889010</partinfo>).
-In absence of progesterone it can bind to its binding site (<partinfo>BBa_K3889030</partinfo>) and repress transcription of the gene.Now as progesterone enters the system SRTF1 binds to progesterone leaving its binding site free for the transcription machinery to start working. This results in a progesterone inducible gene expression.
+In absence of progesterone it can bind to its binding site (<partinfo>BBa_K3889030</partinfo>) and repress transcription of the gene. Now as progesterone enters the system SRTF1 binds to progesterone leaving its binding site free for the transcription machinery to start working. This results in a progesterone inducible gene expression.
+mcherry was linked with the help of P2A linker (<partinfo>BBa_K3889069</partinfo>)in order to coexpress it with SRTF1 and check protein expression
 ===Usage and Biology===